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Jonas Ohlsson
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fix typos. use https where available.
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wms.md

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@@ -34,7 +34,7 @@ The choice of shotgun or 16S approaches is usually dictated by the nature of the
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* [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
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* [Kraken](https://ccb.jhu.edu/software/kraken/)
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* [Bracken](http://ccb.jhu.edu/software/bracken/)
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* [Bracken](https://ccb.jhu.edu/software/bracken/)
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* [R](https://www.r-project.org/)
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* [Pavian](https://github.com/fbreitwieser/pavian)
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```
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We'll use FastQC to check the quality of our data. FastQC can be downloaded and
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ran on a Windows or LINUX computer without installation. It is available [here](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
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run on a Windows or Linux computer without installation. It is available [here](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/)
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Start FastQC and select the fastq file you just downloaded with `file -> open`
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What do you think about the quality of the reads? Do they need trimming? Is there still adapters
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What do you think about the quality of the reads? Do they need trimming? Are there still adapters
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present? Overrepresented sequences?
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Alternatively, run fastqc on the command-line:
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`fastqc *.fastq.gz`
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If the quality appears to be that good, it's because it was probably the cleaned reads that were deposited into SRA.
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If the quality appears to be good, it's because it was probably the cleaned reads that were deposited into SRA.
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We can directly move to the classification step.
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### Taxonomic Classification
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Kraken aims to achieve high sensitivity and high speed by utilizing exact alignments of k-mers and a novel classification algorithm (sic).
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In short, kraken uses a new approach with exact k-mer matching to assign taxonomy to short reads. It is *extremely* fast compared to traditional
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approaches (i.e. Blast)
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approaches (i.e. BLAST).
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By default, the authors of kraken built their database based on RefSeq Bacteria, Archea and Viruses. We'll use it for the purpose of this tutorial.
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**NOTE: The database may have been installed already! Ask your instructor!**
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```bash
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# You might not need this step (example if you're working on Uppmax!)
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# You might not need this step (for example if you're working on Uppmax!)
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wget https://ccb.jhu.edu/software/kraken/dl/minikraken.tgz
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tar xzf minikraken.tgz
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$KRAKEN_DB=minikraken_20141208

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