@@ -34,7 +34,7 @@ The choice of shotgun or 16S approaches is usually dictated by the nature of the
3434
3535* [ FastQC] ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ )
3636* [ Kraken] ( https://ccb.jhu.edu/software/kraken/ )
37- * [ Bracken] ( http ://ccb.jhu.edu/software/bracken/)
37+ * [ Bracken] ( https ://ccb.jhu.edu/software/bracken/)
3838* [ R] ( https://www.r-project.org/ )
3939* [ Pavian] ( https://github.com/fbreitwieser/pavian )
4040
@@ -49,17 +49,17 @@ cd wms
4949```
5050
5151We'll use FastQC to check the quality of our data. FastQC can be downloaded and
52- ran on a Windows or LINUX computer without installation. It is available [ here] ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ )
52+ run on a Windows or Linux computer without installation. It is available [ here] ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ )
5353
5454Start FastQC and select the fastq file you just downloaded with ` file -> open `
55- What do you think about the quality of the reads? Do they need trimming? Is there still adapters
55+ What do you think about the quality of the reads? Do they need trimming? Are there still adapters
5656present? Overrepresented sequences?
5757
5858Alternatively, run fastqc on the command-line:
5959
6060` fastqc *.fastq.gz `
6161
62- If the quality appears to be that good, it's because it was probably the cleaned reads that were deposited into SRA.
62+ If the quality appears to be good, it's because it was probably the cleaned reads that were deposited into SRA.
6363We can directly move to the classification step.
6464
6565### Taxonomic Classification
@@ -68,14 +68,14 @@ We can directly move to the classification step.
6868Kraken aims to achieve high sensitivity and high speed by utilizing exact alignments of k-mers and a novel classification algorithm (sic).
6969
7070In short, kraken uses a new approach with exact k-mer matching to assign taxonomy to short reads. It is * extremely* fast compared to traditional
71- approaches (i.e. Blast)
71+ approaches (i.e. BLAST).
7272
7373By default, the authors of kraken built their database based on RefSeq Bacteria, Archea and Viruses. We'll use it for the purpose of this tutorial.
7474
7575** NOTE: The database may have been installed already! Ask your instructor!**
7676
7777``` bash
78- # You might not need this step (example if you're working on Uppmax!)
78+ # You might not need this step (for example if you're working on Uppmax!)
7979wget https://ccb.jhu.edu/software/kraken/dl/minikraken.tgz
8080tar xzf minikraken.tgz
8181$KRAKEN_DB =minikraken_20141208
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