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Copy file name to clipboardExpand all lines: qc.md
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@@ -22,8 +22,10 @@ FastQC has a graphical interface and can be downloaded and ran on a Windows or L
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However, FastQC is also available as a command line utility on the training server you are using. You can load the module and execute the program as follow:
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`module load fastqc`
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`fastqc $read1 $read2`
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```
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module load fastqc
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fastqc $read1 $read2
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```
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which will produce both a .zip archive containing all the plots, and a html document for you to look at the result in your browser.
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@@ -94,3 +96,7 @@ What did the trimming do to the per-base sequence quality, the per sequence qual
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What is the sequence duplication levels graph about? Why should you care about a high level of duplication, and why is the level of duplication very low for this data?
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Based on the FastQC report, there seems to be a population of shorter reads that are technical artefacts. We will ignore them for now as they will not interfere with our analysis.
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## Extra exercises
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Perform quality control on the extra datasets given by your instructors
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