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Liver digestion protocol v2.0 — merge with AG Surace SOP #001 #4

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41846d3
Update liver digestion protocol to v2.0 (merge with AG Surace SOP #001)
Eomesodermin Aug 5, 2026
f4553e4
Update LCMV infection, IEL isolation, and liver digestion protocols
Eomesodermin Aug 21, 2026
7248c03
Merge branch 'main' into update-liver-digestion-v2
Eomesodermin Aug 21, 2026
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14 changes: 2 additions & 12 deletions .gitignore
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/.quarto/
**/*.quarto_ipynb
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CLAUDE.md
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Expand Down Expand Up @@ -427,9 +427,9 @@ <h1 class="title">Buffers</h1>
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<span class="listing-protocol_id">BUF-BHK-PROP-001</span>
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Expand All @@ -459,9 +459,9 @@ <h1 class="title">Buffers</h1>

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<span class="listing-protocol_id">BUF-FACS-MACS-v1.1</span>
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<span class="listing-protocol_id">BUF-FREEZE-MIX-2x-001</span>
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<span class="listing-version">v1.1</span>
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<span class="listing-protocol_id">BUF-MUS-LIV-DIG-COL-IV-001</span>
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<a href="../../protocols_src/buffers/buf_mus_liver_digestion_col_IV.html" class="title listing-title">Mouse Liver Digestion Buffer with Collagenase IV</a>
<a href="../../protocols_src/buffers/buf_mus_liver_digestion.html" class="title listing-title">Mouse Liver Digestion Buffer</a>
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<span class="listing-date">Nov 21, 2025</span>
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<span class="listing-protocol_id">BUF-MUS-NK-MED-001</span>
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<td>
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Expand All @@ -555,25 +555,25 @@ <h1 class="title">Buffers</h1>

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<span class="listing-protocol_id">BUF-PERC-37-001</span>
<span class="listing-protocol_id">BUF-PERC-37</span>
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<a href="../../protocols_src/buffers/buf_percoll_37pct.html" class="title listing-title">37% Percoll Solution</a>
<a href="../../protocols_src/buffers/buf_percoll_37pct.html" class="title listing-title">37% Percoll Solution (= 33% final Percoll)</a>
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<td>
<span class="listing-version">v1.2</span>
<span class="listing-version">v2.0</span>
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Expand All @@ -587,9 +587,9 @@ <h1 class="title">Buffers</h1>

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Expand All @@ -603,9 +603,9 @@ <h1 class="title">Buffers</h1>

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<span class="listing-protocol_id">BUF-RBC-HYB-v1.0</span>
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Expand All @@ -619,25 +619,25 @@ <h1 class="title">Buffers</h1>

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<span class="listing-protocol_id">BUF-TCELL-MED-001</span>
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<td>
<a href="../../protocols_src/buffers/buf_Tcell_med.html" class="title listing-title">T Cell Medium (RPMI + 10% FCS + Supplements)</a>
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<span class="listing-version">v1.1</span>
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<span class="listing-protocol_id">BUF-VERO-PROP-001</span>
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<td>
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Expand Up @@ -459,7 +459,7 @@ <h1 class="title">Mouse protocols</h1>

</tr>

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<td>
<span class="listing-protocol_id">MUS-IEL-ISO</span>
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Expand All @@ -475,18 +475,18 @@ <h1 class="title">Mouse protocols</h1>

</tr>

<tr data-index="3" data-listing-date-sort="1767913200000" data-listing-file-modified-sort="1776065733477" data-listing-date-modified-sort="NaN" data-listing-reading-time-sort="6" data-listing-word-count-sort="1066" data-listing-title-sort="LCMV Infection Models (WE, Clone 13, Docile, Armstrong)">
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<td>
<span class="listing-protocol_id">MUS-INF-LCMV</span>
</td>
<td>
<a href="../../protocols_src/mouse/prot_mus_lcmv_infection.html" class="title listing-title">LCMV Infection Models (WE, Clone 13, Docile, Armstrong)</a>
</td>
<td>
<span class="listing-version">v1.0</span>
<span class="listing-version">v1.1</span>
</td>
<td>
<span class="listing-date">Jan 9, 2026</span>
<span class="listing-date">Apr 21, 2026</span>
</td>

</tr>
Expand Down Expand Up @@ -555,18 +555,18 @@ <h1 class="title">Mouse protocols</h1>

</tr>

<tr data-index="8" data-listing-date-sort="1764111600000" data-listing-file-modified-sort="1775656332320" data-listing-date-modified-sort="NaN" data-listing-reading-time-sort="14" data-listing-word-count-sort="2736" data-listing-title-sort="Mouse Liver Digestion to Single-Cell Suspension">
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<td>
<span class="listing-protocol_id">MUS-LIV-DIG</span>
</td>
<td>
<a href="../../protocols_src/mouse/prot_mus_liver_digestion.html" class="title listing-title">Mouse Liver Digestion to Single-Cell Suspension</a>
</td>
<td>
<span class="listing-version">v1.2</span>
<span class="listing-version">v2.0</span>
</td>
<td>
<span class="listing-date">Nov 26, 2025</span>
<span class="listing-date">Aug 5, 2026</span>
</td>

</tr>
Expand Down Expand Up @@ -683,18 +683,18 @@ <h1 class="title">Mouse protocols</h1>

</tr>

<tr data-index="16" data-listing-date-sort="1774479600000" data-listing-file-modified-sort="1776067352533" data-listing-date-modified-sort="NaN" data-listing-reading-time-sort="20" data-listing-word-count-sort="3882" data-listing-title-sort="xCELLigence Real-Time Cytotoxicity Assay for LCMV-Infected Fibroblasts">
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<td>
<span class="listing-protocol_id">MUS-XCELL-LCMV</span>
</td>
<td>
<a href="../../protocols_src/mouse/prot_xcelligence_lcmv_cytotoxicity.html" class="title listing-title">xCELLigence Real-Time Cytotoxicity Assay for LCMV-Infected Fibroblasts</a>
</td>
<td>
<span class="listing-version">v1.4</span>
<span class="listing-version">v1.6</span>
</td>
<td>
<span class="listing-date">Mar 26, 2026</span>
<span class="listing-date">Apr 15, 2026</span>
</td>

</tr>
Expand Down
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